Photosynthetic characteristics and tolerance to photo-oxidation of transgenic rice expressing C(4) photosynthesis enzymes.
作者： Demao Jiao , Xueqing Huang , Xia Li , Wei Chi , Tingyun Kuang
关键词: Photosynthesis 、 Photosynthetic capacity 、 Biochemistry 、 RuBisCO 、 Pyruvate carboxylase 、 Plant physiology 、 Light intensity 、 Phosphoenolpyruvate carboxylase 、 Genetically modified rice 、 Biology
摘要: The photosynthetic characteristics of four transgenic rice lines over-expressing NADP-malic enzyme (ME), and maize phosphoenolpyruvate carboxylase (PC), pyruvate,orthophosphate dikinase (PK), PC+PK (CK) were investigated using outdoor-grown plants. Relative to untransformed wild-type (WT) rice, PC exhibited high activity (25-fold increase) enhanced carbonic anhydrase (more than two-fold increase), while the ribulose-bisphosphate carboxylase/oxygenase (Rubisco) its kinetic property not significantly altered. plants also showed a higher light intensity for saturation photosynthesis, CO(2) uptake rate carboxylation efficiency, slightly reduced compensation point. In addition, chlorophyll fluorescence analysis indicates that are more tolerant photo-oxidative stress, due capacity quench excess energy via photochemical non-photochemical means. Furthermore, CK produced 22-24% grains WT Taken together, these results suggest expression C(4) photosynthesis enzymes in C(3) plant, can improve with tolerance photo-oxidation.
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