Essential developmental biology: a practical approach.

摘要: Part 1 Basic techniques - obtaining and handling embryos: drosophila embryos, Zandy Forbes Philip Ingham nematode Lois G. Edgar William B. Wood embryos larvae of invertebrate deuterostomes, Nicholas D. Holland Linda Z. zebrafish Rachel M. Warga Xenopus, Ariel Ruis I. Altaba avian Claudio Stern post-implantation mammalian Gillian Morriss-Kay simple tips for photomicrography Stern. 2 Experimental embryology: fate maps cell lineage analysis, Ruiz et al micro-injection into transplantation in xenopus, chick limb buds, Cheryll tickle nuclear transplantation, John Gordon laser ablation cells, Judith Eisen al. 3 Cellular techniques: studying movements vivo by time-lapse video microscopy, Peter V. Thorogood Dee Amanze induction assays culture neural crest Elisabeth Dupin Nicole Le Douarin embryonic stem cells gene targeting, Elizabeth J. Robertson Gail R. Martin introduction genes using retroviral vectors, Jack Price. 4 Molecular immonocytochemistry material, RNAase protection assays, cDNA library construction, Akira Kakizuka analysis expression reverse transcription polymerase chain reaction, Koopman cloning the W.H. situ hybridization, David Wilkinson. Appendices: stage tables suppliers specialist items.