Serotype and genotype analysis of dengue virus by sequencing followed by phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan

作者: Zareen Fatima , Muhammad Idrees , Mohammad A Bajwa , Zarfishan Tahir , Obaid Ullah

DOI: 10.1186/1471-2180-11-200

关键词: BiologySerotypeGenotypePhylogenetic treeParasitologyVirologyDengue hemorrhagic feverMicrobiologyOutbreakDengue virusPolymerase chain reaction

摘要: Since the first reported outbreak of dengue hemorrhagic fever in Pakistan, several mini outbreaks have erupted region. Dengue virus serotype 3 (DEN-3) was documented 2005 Karachi. Reports show that is prevalent Lahore since 2008. Serotype 2 (DEN-2) major circulating Pakistan as it 1994. We conducted a detailed study three infection occurred years 2007, 2008 and 2009 by using molecular techniques such PCR nucleotide sequencing C-prM gene junction virus. Through analysis 114 serum samples collected over period (2007-2009), total 20 patients were found to be infected with In year four positive for one sample DEN-3. 2008, five had concurrent serotypes DEN-2 DEN-3 while only DEN-2. 2009, six only. Our showed dominant during (2007-2009). The other present Genotypes subtype IV III, respectively.

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