Peptides selected for binding to clotted plasma accumulate in tumor stroma and wounds.

摘要: Screening of a phage library for peptides that bind to clotted plasma in the presence liquid yielded two cyclic decapeptides, CGLIIQKNEC (CLT1) and CNAGESSKNC (CLT2). When injected intravenously into mice bearing various types tumors, fluorescein-conjugated CLT accumulated fibrillar meshwork extracellular compartment but were not detectable other tissues tumor-bearing mice. The tumor homing both was strongly reduced after coinjection with unlabeled CLT2, indicating recognize same binding site. peptide fluorescence colocalized staining fibrin(ogen) present extravascular tissues. did home tumors grown fibrinogen-null or lack fibronectin. also at sites injury arteries, skeletal muscle, skin. We conclude fibrin-fibronectin complexes formed by clotting proteins have leaked space lesions. These may be useful targeting diagnostic therapeutic materials injured