Genetic and functional complementation of the HSV1 UL27 gene and gB glycoprotein by simian α-herpesvirus homologs

摘要: Utilizing co-transfection of DNA from glycoprotein gB− strain HSV1 and cloned fragments several simian α-herpesviruses containing the UL26, UL27 (gB glycoprotein), UL28 gene homologs, replication-compe-tent recombinant viruses were produced. Genetic analysis one HSVI/SAff8 (HSV1/SgB) demonstrated presence SAff8 comprising entire (gB) parts UL26 ORFs in an otherwise genome. The was shown to express gB p40 proteins (UL27 & UL26.5 products, respectively); all other indistinguishable those HSV1. behaved like gB-specific virus neutralization cell surface antibody binding assays, while plaque morphology replication kinetics very similar Despite its overwhelming genetic constitution, displayed a pathogenic phenotype mice different parental While produced corneal disease ocularly infected readily spread nervous system, HSV1/SgB markedly impaired both respects. These results demonstrate functional equivalency cercopithecine monkey glycoproteins genes (including transcriptional regulatory elements) HSV1, funtional nature HSV1/SAff8 chimeric genes/proteins, that UL28, and/or may effect pathogenicity