Deregulated miRNAs in Hereditary Breast Cancer Revealed a Role for miR-30c in Regulating KRAS Oncogene

摘要: Aberrant miRNA expression has been previously established in breast cancer and clinical relevance. However, no studies so far have defined miRNAs deregulated hereditary tumors. In this study we investigated the role of tumors comparing with normal tissue. Global profiling using Exiqon microarrays was performed on 22 15 non-tumoral tissues. We identified 19 differentially expressed, most them down-regulated An important proportion were commonly sporadic Under-expression these validated by qRT-PCR additional 18 their tissue counterparts. Pathway enrichment analysis revealed that collectively targeted a number genes belonging to signaling pathways such as MAPK, ErbB, mTOR, those regulating cell motility or adhesion. silico prediction detected KRAS oncogene target several miRNAs. particular, experimentally miR-30c target. Luciferase assays confirmed binds 3′UTR transcripts pre-miR-30c mRNA protein. Furthermore, overexpression inhibited proliferation cells. Our results identify associated cancer, well miss-expressed tumors, suggesting common underlying mechanisms tumor progression. addition, provide evidence is miR-30c, suppresses growth potentially through inhibition signaling.