The micronuclear gene encoding a putative aminoacyl-tRNA synthetase cofactor of the ciliate Euplotes octocarinatus is interrupted by two sequences that are removed during macronuclear development
作者： M. Tan , K. Heckmann , C. Brünen-Nieweler
DOI: 10.1016/S0378-1119(99)00146-8
关键词: Aminoacyl tRNA synthetase 、 Coding region 、 Peptide sequence 、 DNA 、 Genetics 、 Molecular biology 、 Macronucleus 、 Biology 、 Direct repeat 、 Complementary DNA 、 Gene
摘要: The micronuclear gene of the ciliated protozoan Euplotes octocarinatus (Eo) syngen 1 encoding putative aminoacyl-tRNA synthetase cofactor (ARCE), as well its macronuclear version and corresponding cDNA, were amplified sequenced. Analyses sequences revealed that contains two (430 625bp long) are missing in this gene. These called 'internal eliminated sequences' (IESs) appear to occur all ciliates. IESs located coding region One IES is flanked by a pair dinucleotide 5'-TA-3' direct repeats other one hepta-nucleotide 5'-TTACTGA-3' repeats. Inside IESs, several sequence found. DNA molecule carrying 1517bp long shows characteristics typical chromosomes hypotrichous Copy number determination only about 750 copies per macronucleus. deduced protein 441-amino-acid (aa) polypeptide with molecular mass 50kDa. It shares conserved endothelial monocyte-activating II (EMAP II)-like carboxyl-terminal domain hydrophilic central containing KEKE-motif group proteins associated synthetases tRNAs.
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