In vitro antioxidant activity and polyphenolic content of a polyherbal tea and its constituents.
作者： V. Paddy , J. Tonder , V. Steenkamp
关键词: Officinalis 、 DPPH 、 Salvia officinalis 、 Chemistry 、 Traditional medicine 、 Trolox 、 ABTS 、 Agathosma betulina 、 Thymus vulgaris 、 Flavonoid
摘要: Aims: Type 2 diabetes mellitus (T2DM) is associated with debilitating co-morbidities, mainly due to chronic hyperglycaemia-induced damage which mediated by excess reactive oxygen species (ROS). Plant preparations have been shown contain polyphenolic compounds that function as antioxidants. Various herbal teas sold treatment of mellitus. The aim this study was evaluate a commercial, polyherbal tea, Diabetea and its constituents: Achillea millefolium L., Agathosma betulina Bartl. & Weidl., Salvia officinalis. Taraxacum officinalis Thymus vulgaris. Trigonella foenum-graecum L. Urtica urens assess their antioxidant content. Methodology: polyphenol-linked cell-free cell-based activities hot water (HW) dichloromethane (DCM) extracts constituents were evaluated for ABTS ●+ DPPH ● radical scavenging ability ROS activity using 2',7'dichlorodihydrofluorescein diacetate (DCFH-DA) in Ea.hy926 endothelial cells, respectively. phenolic flavonoid content also assessed. Results: All HW rich polyphenols. T. vulgaris contained the highest amount flavonoids (760.2±1.3 mg rutin equivalent (RE)/g) all extracts, followed S. Original Research Article Paddy et al.; EJMP, 7(4): 215-226, 2015; no.EJMP.2015.084 216 (491.7±12.5 RE/g). most active against (~600.0 trolox equivalents (TE)/g activity) (535.0 TE/g activity). vulgaris, U. significantly (p<0.05) mitigated cellular ROS, whereas none DCM had effect. Diabetea, A. betulina, (exception betulina) significant intracellular pro-oxidant activity. correlated (r=0.98) Conclusion: exerted strong greater than extracts. bioactive exert vitro antidiabetic potential. Identification responsible warranted.
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