A simple quantitative HPLC assay for ifosfamide in biological fluids.
作者： Jennifer M. Margison , P. M. Wilkinson , T. Cerny , N. Thatcher
关键词: Gas chromatography 、 Chromatography 、 High-performance liquid chromatography 、 Chemistry 、 Ifosfamide 、 Standard curve 、 Biological specimen 、 Reversed-phase chromatography 、 Pharmacokinetics 、 Detection limit
摘要: A high performance liquid chromatography method is described for measuring Ifosfamide (I) in human serum. This involves solvent extraction, reverse phase HPLC and UV detection at 190 nm. Standard curves of peak height × detector sensitivity versus I concentration serum were linear with a lower limit 100 ng/ml. Authentic 14C-labelled cochromatographed standard found from treated patients. The concentration-time determined by both gas indistinguishable. We conclude that this suitable determining pharmacokinetics biological specimens.
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