Mechanical properties of chicken embryo mesoderm during somite formation (Conference Presentation)

摘要: The majority of tissue related diseases are known to alter structure. During the last 10 years, increasing efforts have been put into development new techniques that could provide in vivo information on morphology. Optical coherence tomography (OCT) is structural situ. However, there also a strong demand evaluate mechanical properties biological tissues vivo. To address this need, we combined microindentation with non-invasive OCT imaging determine spatiotemporal distributions and formaldehyde fixed chicken embryos. use allows us quantify changes morphology localize indentation at specific regions. measure viscoelastic living tissue, tests simultaneously performed HH8-HH12 embryos using cantilever based force transducer. After performing live indentation, probed embryo. same general contrasts elasticity between different histological regions were found, but average value was found be higher for sample. Furthermore, technique it possible follow remodeling elastic during embryonic development, investigate correlations local cell migration differentiation. This method applicable wide variety samples can insight better understand link response its structure, compare diseased healthy one.