The Drosophila esc and E(z) proteins are direct partners in polycomb group-mediated repression
作者： Clark A. Jones , Joyce Ng , Aidan J. Peterson , Kelly Morgan , Jeffrey Simon
关键词: Immunoprecipitation 、 Psychological repression 、 Molecular biology 、 Biology 、 Enhancer 、 Nuclear protein 、 Plasma protein binding 、 Drosophila Protein 、 Repressor 、 Conserved sequence
摘要: The extra sex combs (esc) and Enhancer of zeste [E(z)] proteins are members the Drosophila Polycomb group (Pc-G) transcriptional repressors. Here we present evidence for direct physical interaction between esc E(z) using yeast two-hybrid in vitro binding assays. In addition, coimmunoprecipitation from embryo extracts demonstrates association vivo. We have delimited esc-binding domain to an N-terminal 33-amino-acid region. Furthermore, demonstrate that site-directed mutations protein previously shown impair function vivo disrupt esc-E(z) interactions vitro. also show heed EZH1 proteins, which human homologs E(z), respectively. These results suggest molecular partnership has been conserved evolution. Previous studies suggested is primarily involved early stages Pc-G-mediated silencing during embryogenesis. However, continuously required order maintain chromosome by other Pc-G proteins. light these earlier observations data presented here, discuss how complexes may contribute Pc-G.
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