Transverse Diffusion of Laminar Flow Profiles To Produce Capillary Nanoreactors
作者： Victor Okhonin , Xin Liu , Sergey N. Krylov
DOI: 10.1021/AC0508806
关键词: Diffusion 、 Laminar flow 、 Transverse plane 、 Chemical kinetics 、 Analytical chemistry 、 Laminar flow reactor 、 Capillary action 、 Chemistry 、 Flow (psychology) 、 Mixing (process engineering) 、 Mechanics
摘要: We introduce transverse diffusion of laminar flow profiles (TDLFP), the first generic method for mixing two or more reactants inside capillaries. Conceptually, solutions are injected capillary by pressure as a series consecutive plugs. Due to nature capillary, nondiffused plugs have parabolic with predominantly longitudinal interfaces between them. After injection, mixed diffusion; does not contribute mixing. To prove principle, we used TDLFP mix reactantsan enzyme and its substrate. TDLFP, incubated reaction mixtures different periods time measured kinetics. found that proceeded in time- concentration-dependent fashion, thus confirming were TDLFP. Remarkably, experimental kinetics only qualitative agreement but also good quantitative agreem...
acs.org
unirioja.es
sci-hub.se 参考文章(16)
Christopher T. Culbertson, James W. Jorgenson, Flow Counterbalanced Capillary Electrophoresis Analytical Chemistry. ,vol. 66, pp. 955- 962 ,(1994) , 10.1021/AC00079A006
K. Kimura, Phosphorylation and Activation of 13S Condensin by Cdc2 in Vitro Science. ,vol. 282, pp. 487- 490 ,(1998) , 10.1126/SCIENCE.282.5388.487
Upma Sharma, Nathaniel J. Gleason, Jeffrey D. Carbeck, Diffusivity of solutes measured in glass capillaries using Taylor's analysis of dispersion and a commercial CE instrument. Analytical Chemistry. ,vol. 77, pp. 806- 813 ,(2005) , 10.1021/AC048846Z
Sigrid Van Dyck, Stijn Vissers, Ann Van Schepdael, Jos Hoogmartens, Kinetic study of angiotensin converting enzyme activity by capillary electrophoresis after in-line reaction at the capillary inlet. Journal of Chromatography A. ,vol. 986, pp. 303- 311 ,(2003) , 10.1016/S0021-9673(02)01995-7
K. V. Sharp, R. J. Adrian, Transition from laminar to turbulent flow in liquid filled microtubes Experiments in Fluids. ,vol. 36, pp. 741- 747 ,(2004) , 10.1007/S00348-003-0753-3
Victor Okhonin, Maxim Berezovski, Sergey N. Krylov, Sweeping capillary electrophoresis: a non-stopped-flow method for measuring bimolecular rate constant of complex formation between protein and DNA. Journal of the American Chemical Society. ,vol. 126, pp. 7166- 7167 ,(2004) , 10.1021/JA0481124
Maxim Berezovski, Andrei Drabovich, Svetlana M. Krylova, Michael Musheev, Victor Okhonin, Alexander Petrov, Sergey N. Krylov, Nonequilibrium Capillary Electrophoresis of Equilibrium Mixtures: A Universal Tool for Development of Aptamers Journal of the American Chemical Society. ,vol. 127, pp. 3165- 3171 ,(2005) , 10.1021/JA042394Q
Atsushi Taga, Susumu Honda, Derivatization at capillary inlet in high-performance capillary electrophoresis Its reliability in quantification Journal of Chromatography A. ,vol. 742, pp. 243- 250 ,(1996) , 10.1016/0021-9673(96)00250-6
R. Kennedy, M. Oates, B. Cooper, B Nickerson, J. Jorgenson, Microcolumn separations and the analysis of single cells Science. ,vol. 246, pp. 57- 63 ,(1989) , 10.1126/SCIENCE.2675314
Maxim Berezovski, Sergey N. Krylov, Using DNA-binding proteins as an analytical tool. Journal of the American Chemical Society. ,vol. 125, pp. 13451- 13454 ,(2003) , 10.1021/JA037186X