Two forms of parathyroid hormone-like adenylate cyclase-stimulating protein derived from tumors associated with humoral hypercalcemia of malignancy
作者： Andrew F. Stewart , William J. Burtis , Terence Wu , Douglas Goumas , Arthur E. Broadus
关键词: Pathogenesis 、 Parathyroid hormone 、 Adenylate kinase 、 Endocrinology 、 Pheochromocytoma 、 Malignancy 、 Isoelectric focusing 、 Breast carcinoma 、 Immunohistochemistry 、 Cancer research 、 Internal medicine 、 Chemistry
摘要: Tumors associated with humoral hypercalcemia of malignancy (HHM) contain parathyroid hormone-like adenylate cyclase-stimulating proteins (hACSPs). We previously purified a 17,000 MW hACSP from an HHM-associated breast carcinoma. This report describes the characterization hACSPs three additional tumors: two typical tumors (squamous carcinomas) and third unusual tumor type (pheochromocytoma). Each was extracted in acid-urea/ethanol-sodium chloride, activity (ACSA) examined following reverse-phase size-exclusion HPLC isoelectric focusing. contained high molecular weight form ACSA which co-eluted carcinoma each separation procedures. also lower form(s) (6,000-9,000 weight). Both forms were inhibited by Nle8,18Tyr34bPTH(3-34) amide. The not changed to reducing agent. Some hACSP, one another 6,000-9,000, appears be amino-terminal cleavage product larger protein.
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