Novel phosphorylation of cardiac troponin i as a monitor for cardiac injury
作者： Ger Stienen , Eyk Jennifer E Van , Der Velden Jolanda Van , Anne M Murphy , Pingbo Zhang
DOI:
关键词: Biomarker (medicine) 、 Cardiology 、 Internal medicine 、 Pharmacology 、 Heart failure 、 Medicine 、 Antibody 、 Cardiac troponin 、 Threonine 、 Serine 、 Phosphorylation 、 Tyrosine
摘要: This invention relates to novel phosphorylation sites in cardiac Troponin I that are associated with the onset of heart failure. The sites, i.e., serine 5, tyrosine 26, threonine 51, 166, 181 and/or 199, can be used as biomarkers for (i) identifying subjects at risk development failure, (ii) treating having a higher than normal level biomarker, and (iii) monitoring therapy subject Also described antibodies, reagents, kits carrying out method present invention.
lens.org 参考文章(21)
R. A. Reisfeld, S. Sell, Monoclonal antibodies and cancer therapy UCLA symposium on molecular and cellular biology. Annual meeting. 14. pp. 33- 74 ,(1985)
Jian-Ping Jin, Methods for treating cardiac disease by modifying an N-terminal domain of troponin I ,(2009)
Ninetta Buscemi, Jennifer E. Van Eyk, Michelle K. Quick, Jeremy A. C. Simpson, Isolated post-translationally modified proteins for monitoring and diagnosing muscle damage ,(2003)
David Lane, Ed Harlow, Antibodies: A Laboratory Manual ,(1988)
Lisa J. Garrard, Dennis J. Henner, James A. Wells, Henry B. Lowman, Steven Bass, David J. Matthews, Ronald Greene, Enrichment method for variant proteins having altered binding properties, M13 phagemids, and growth hormone variants ,(1991)
Rebekah L. Gundry, Melanie Y. White, Christopher I. Murray, Lesley A. Kane, Qin Fu, Brian A. Stanley, Jennifer E. Van Eyk, Preparation of Proteins and Peptides for Mass Spectrometry Analysis in a Bottom-Up Proteomics Workflow Current protocols in molecular biology. ,vol. 90, ,(2009) , 10.1002/0471142727.MB1025S88
David Bar-Or, Gregory W. Thomas, Raphael Bar-Or, Leonard Rael, James V. Winkler, Diagnostic potential of phosphorylated cardiac troponin I as a sensitive, cardiac-specific marker for early acute coronary syndrome: Preliminary report Clinica Chimica Acta. ,vol. 362, pp. 65- 70 ,(2005) , 10.1016/J.CCCN.2005.05.015
Andrew E. Messer, Clare E. Gallon, William J. McKenna, Cristobal G. Dos Remedios, Steven B. Marston, The use of phosphate-affinity SDS-PAGE to measure the cardiac troponin I phosphorylation site distribution in human heart muscle. Proteomics Clinical Applications. ,vol. 3, pp. 1371- 1382 ,(2009) , 10.1002/PRCA.200900071
Geoffrey Walter Grigg, James David Marks, Gregory Paul Winter, Hendricus Renerus Jacobus Mattheus Hoogenboom, Andrew David Griffiths, John McCafferty, Production of anti-self bodies from antibody segment repertories and displayed on phage ,(2000)
Nils Lonberg, Robert M. Kay, Transgenic non-human animals for producing heterologous antibodies ,(1992)