Progress toward characterization of the group A Streptococcus metagenome: Complete genome sequence of a macrolide-resistant serotype M6 strain
作者： David J. Banks , Stephen F. Porcella , Kent D. Barbian , Stephen B. Beres , Lauren E. Philips
DOI: 10.1086/422697
关键词: Streptococcus pyogenes 、 Biology 、 Lysogenic cycle 、 Microbiology 、 Genetics 、 Whole genome sequencing 、 Genome 、 Nucleic acid sequence 、 Transposable element 、 Prophage 、 Gene
摘要: We describe the genome sequence of a macrolide-resistant strain (MGAS10394) serotype M6 group A Streptococcus (GAS). The is 1,900,156 bp in length, and 8 prophage-like elements or remnants compose 12.4% chromosome. 8.3-kb prophage remnant encodes SpeA4 variant streptococcal pyrogenic exotoxin A. MGAS10394 contains chimeric genetic element composed genes transposon encoding mefA gene conferring macrolide resistance. This also has novel surface-exposed protein (designated "R6 protein"), with an LPKTG cell-anchor motif located at carboxyterminus. Surface expression this was confirmed by flow cytometry. Humans GAS pharyngitis caused strains had antibody against R6 present convalescent, but not acute, serum samples. Our studies add to theme that prophage-encoded extracellular proteins contribute host-pathogen interactions strain-specific fashion.
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