Differentiation of infection from vaccination in foot-and-mouth disease by the detection of antibodies to the non-structural proteins 3D, 3AB and 3ABC in ELISA using antigens expressed in baculovirus
作者： K. J. Sørensen , K. G. Madsen , E. S. Madsen , J. S. Salt , J. Nqindi
关键词: Serology 、 Aphthovirus 、 Virology 、 Antibody 、 Population 、 Vaccination 、 Biology 、 Virus 、 Antigen 、 Serotype
摘要: The baculovirus expression system was found to be efficient at expressing the 3D, 3AB and 3ABC non-structural proteins (NSP) of foot-and-mouth disease virus (FMDV) as antigens recognised by immune sera in ELISA. ELISA's using detected antibodies from day 8 10 after experimental infection susceptible cattle sheep remained seropositive for more than 395 days. against any seven serotypes FMDV. 3D ELISA specific precise sensitive established which measure antibody structural proteins. assay may used a resource saving alternative detection serotypes. were also precise. FMDV infected could differentiated those that had been merely vaccinated they gave positive result both ELISA's. Two results tests, suggesting ELISA's, but not might represent reliable means detecting population.
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de Mello Pa, Beck E, Gomes I, Neitzert E, Bergmann Ie, Diagnosis of persistent aphthovirus infection and its differentiation from vaccination response in cattle by use of enzyme-linked immunoelectrotransfer blot analysis with bioengineered nonstructural viral antigens. American Journal of Veterinary Research. ,vol. 54, pp. 825- 831 ,(1993)
B H Robertson, M J Grubman, G N Weddell, D M Moore, J D Welsh, T Fischer, D J Dowbenko, D G Yansura, B Small, D G Kleid, Nucleotide and amino acid sequence coding for polypeptides of foot-and-mouth disease virus type A12. Journal of Virology. ,vol. 54, pp. 651- 660 ,(1985) , 10.1128/JVI.54.3.651-660.1985
Martin Sw, The evaluation of tests. Canadian journal of comparative medicine. ,vol. 41, pp. 19- 25 ,(1977)
Richard F. Meyer, Gwen D. Babcock, John F.E. Newman, Tom G. Burrage, Kathy Toohey, Juan Lubroth, Fred Brown, Baculovirus expressed 2C of foot-and-mouth disease virus has the potential for differentiating convalescent from vaccinated animals Journal of Virological Methods. ,vol. 65, pp. 33- 43 ,(1997) , 10.1016/S0166-0934(96)02165-9
J. F. Newman, P. G. Piatti, B. M. Gorman, T. G. Burrage, M. D. Ryan, M. Flint, F. Brown, Foot-and-mouth disease virus particles contain replicase protein 3D. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 733- 737 ,(1994) , 10.1073/PNAS.91.2.733
Eva Smedegaard Madsen, Knud Gert Madsen, Jens Nielsen, Merethe Holm Jensen, Jørgen Christian Lei, Per Have, Detection of antibodies against porcine parvovirus nonstructural protein NS1 may distinguish between vaccinated and infected pigs. Veterinary Microbiology. ,vol. 54, pp. 1- 16 ,(1997) , 10.1016/S0378-1135(96)01270-9
H.A. Westbury, Pornchai Chamnanpood, Suchinta Tangchaitrong, W.J. Doughty, Single dilution ELISA for detection of serum antibody to foot-and-mouth disease virus in cattle. Veterinary Microbiology. ,vol. 18, pp. 273- 283 ,(1988) , 10.1016/0378-1135(88)90093-4
J Lubroth, F Brown, Identification of native foot-and-mouth disease virus non-structural protein 2C as a serological indicator to differentiate infected from vaccinated livestock. Research in Veterinary Science. ,vol. 59, pp. 70- 78 ,(1995) , 10.1016/0034-5288(95)90034-9
K.J. Sorensen, R.L. Madekurozwa, P. Dawe, Foot-and-mouth disease: detection of antibodies in cattle sera by blocking ELISA. Veterinary Microbiology. ,vol. 32, pp. 253- 265 ,(1992) , 10.1016/0378-1135(92)90148-M
Ana Rodr�guez, J. Dopazo, J. C. S�iz, F. Sobrino, Immunogenicity of non-structural proteins of foot-and-mouth disease virus: differences between infected and vaccinated swine Archives of Virology. ,vol. 136, pp. 123- 131 ,(1994) , 10.1007/BF01538822