Glucose oxidase immobilization by polyurethane film/foam in dense CO2 environment
作者： Nilay İnoğlu , Defne Kayrak-Talay , Öner Hortaçsu
DOI: 10.1016/J.PROCBIO.2007.12.007
关键词: Glucose oxidase 、 Polymer 、 Polyurethane 、 Supercritical fluid 、 Swelling 、 Biosensor 、 Chemistry 、 Chemical engineering 、 Supercritical carbon dioxide 、 Chromatography
摘要: Abstract Glucose oxidase (GOX) is immobilized by two different polymer matrices (polyurethane (PU) film and foam) in supercritical carbon dioxide (scCO 2 ) environment for an envisioned application biosensor preparations. Effects of immobilization temperature (25–50 °C), pressure (0.1–15 MPa) time (4–72 h) on the GOX activity PU samples are studied. The interactions between CO also experimentally investigated. Maximum surface area enlargements during foaming 12 250% original films almost no swelling/foaming (0.3–4.4% original) observed foams. characteristics positively correlated with effect sample activities. Immobilized activities found to be inversely proportional temperature. Activities obtained prepared at 8, 15 especially 10 MPa 52, 74 165%, respectively, greater than highest value without using process.
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