DNA topoisomerase II is required for the G0-to-S phase transition in Drosophila Schneider cells, but not in yeast.

摘要: We previously reported that DNA topoisomerase II (topo II) is required for the G0-to-S phase transition in mammalian cells [Hossain et al. (2002) ICRF-193, a catalytic inhibitor of topoisomerase II, inhibits re-entry into cell division cycle from quiescent state cells. Genes Cells 7, 285–294]. In this study, we examined whether requirement topo II evolutionarily conserved Drosophila and yeast. topo II, inhibited synthesis Schneider released G0 (stationary) phase, whereas drug did not inhibit M phase. Depletion mRNA by RNA-interference (RNAi) G0-phase resulted significant inhibition after release G0-arrest. yeast temperature-sensitive (ts) mutant, initial occurred at restrictive temperature starvation-induced doubling content was confirmed both flow cytometry fluorescence spectrophotometry. also observed presence ICRF-193. Doubling during spore germination ts mutant as determined These results indicate cells, but