Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells
作者： Jose B. Cibelli , Stevem L. Stice , Paul J. Golueke , Jeff J. Kane , Joseph Jerry
DOI: 10.1038/NBT0798-642
关键词: Cellular differentiation 、 Ectoderm 、 Embryonic stem cell 、 Cell biology 、 Mesoderm 、 Biology 、 Endoderm 、 Embryo 、 Somatic cell 、 Genetics 、 Stem cell
摘要: We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated derivatives the three germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found be chimeric for at least one tissue. experiments demonstrate that somatic can genetically modified then de-differentiated by transfer ES-like opening possibility them differentiation studies human cell therapy.
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