Control of turnip yellows virus : assessing impact on oilseed rape quality traits and dissecting circulative transmission by aphids

摘要: Abstract Turnip yellows virus (TuYV) is one of the most significant viral diseases of oilseed rape and may be main reasons why commercial oilseed crops do not reach their genetic potential. TuYV transmitted by aphids, sap-sucking hemipteroid insects, green peach aphid (GPA) predominant vector. can reduce oilseed yield up to 26% in UK also affect oil quality. Current control measures rely on insecticides; however, changing legislation reduced effectiveness necessitate novel approaches control. In this thesis, impact of TuYV crop was established sources partial resistance aphids were investigated. reduces has a subtle impact seed physiology including small changes fatty acid profiles and glucosinolate content. Furthermore, these appear genotype-dependent and not as result accumulation plant. To learn more about TuYV transmission novel, functional-genomics tool developed silence aphid genes plant-mediated RNA interference (PMRi). Highly specific protein interactions between particles proteins are critical determinants of circulative transmission, process whereby move cell layers. However, components underlying processes poorly understood. As GPA Rack1 protein been implicated transcytosis TuYV particles across gut barrier, PMRi used dissect its role circulative transmission process. This revealed that have direct TuYV acquisition GPA. work further demonstrates potential postgenomics tool similar insects, but means and/or virus These contrasting research strategies provided two-pronged approach towards improving