High-affinity choline transport in proteoliposomes derived from rat cortical synaptosomes.
作者： E. Meyer , Cooper
关键词: Biochemistry 、 Liposome 、 Chemistry 、 Acetylcholine 、 Choline transporter 、 Phosphatidylcholine 、 Membrane 、 Bilayer 、 Choline transport 、 Synaptic vesicle
摘要: Functional high- and low-affinity choline transport processes from rat cortical plasma membranes were reconstituted in phosphatidylcholine bilayer liposomes. The high-affinity transporter demonstrated a pharmacological profile ion dependency that identical to those of intact synaptosomes. This preparation may be used further characterize and, with appropriate supplementation, investigate the release acetylcholine absence synaptic vesicles.
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