Increase vs. decrease of calcium uptake by isolated heart cells induced by H2O2 vs. HOCl
作者： T. Kaminishi , T. Matsuoka , T. Yanagishita , K. J. Kako
DOI: 10.1152/AJPCELL.1989.256.3.C598
关键词: Biophysics 、 Intracellular 、 Na+/K+-ATPase 、 Hypochlorous acid 、 Myocyte 、 Amiloride 、 Verapamil 、 Chemistry 、 Calcium 、 Digitonin 、 Biochemistry
摘要: Adult rat heart myocytes were labeled rapidly with exogenous [45Ca2+]. Addition of 2.5 mM H2O2 to the cell suspension raised content exchangeable intracellular Ca2+ twofold, whereas addition 1-30 HOCl decreased content. The H2O2-induced increase in was dependent on medium Na+, pH, and temperature but not significantly affected by verapamil, diltiazem, amiloride, or 3-aminobenzamide. [3H]ouabain binding suppressed H2O2, efflux from influenced. An uncoupler, carbonyl cyanide m-chlorophenylhydrazone, reduced content, implying that change directly related ATP depletion. On other hand, accumulation prevented deferoxamine o-phenanthroline. These results suggest inhibited Na+-K+-ATPase, resulting an Na+ concentration stimulation sarcolemmal Na+-Ca2+ exchange activity, which caused a transient net influx into myocytes. By contrast, pool below control levels this action antagonized 1,4-dithiothreitol. accelerated uptake Ca2+-ATPase isolated sarcoplasmic reticular (SR) fraction highly sensitive HOCl. sites, studied permeabilized digitonin, both Thus these inhibits SR pump, observed acceleration decline
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