Proteomic Analysis of Mycoplasma gallisepticum Vaccine Strain F
作者： S.D. Collier , S.L. Branton , J.D. Evans , S.A. Leigh , X.F. Wan
关键词: Membrane protein 、 Chemistry 、 Protein biosynthesis 、 Mycoplasma gallisepticum vaccine 、 Mycoplasma gallisepticum 、 SOSUI 、 Proteome 、 Whole cell lysate 、 Cytosol 、 Biochemistry
摘要: The persistence and displacement abilities of the Mycoplasma gallisepticum vaccine strain F (F-strain) are well documented. Understanding mechanism(s) colonization F-strain will aid in current intervention strategies to diagnose control MG infections poultry. In present study, phase partition liquid chromatography along with electrospray mass spectrometry were used evaluate proteome F-strain. A total 586, 478 339 proteins recognized from whole cell lysate (total protein), aqueous (cytosolic) detergent phases (membrane), respectively. identified database searches then grouped into three categories: (1) membrane found (TM); (2) cytosolic (TC) (3) derived (TMC). There a 93 (33 as putative predicted by SOSUI), 207(13) 79(6) TM, TC TMC, distributed among John Craig Venter Institute (JCVI) categories, majority be involved protein synthesis. Investigation may identification characterization that important host colonization.
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