Reengineering mrna primary structure for enhanced protein production
作者： Vincent P. Mauro , Stephen A. Chappell , Wei Zhou , Gerald M. Edelman
DOI:
关键词: Translation (biology) 、 Computational biology 、 Biology 、 Protein primary structure 、 Untranslated region 、 MiRNA binding 、 Genetics 、 Protein biosynthesis 、 Messenger RNA 、 Ribosomal RNA
摘要: Described herein are rules to modify natural mRNAs or engineer synthetic increase their translation efficiencies. These describe modifications mRNA coding and 3′ UTR sequences intended enhance protein synthesis by: 1) decreasing ribosomal diversion via AUG non-canonical initiation codons in sequences, and/or 2) by evading miRNA-mediated down-regulation eliminating one more miRNA binding sites sequences.
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