Development of anti-HER2 fragment antibody conjugated to iron oxide nanoparticles for in vivo HER2-targeted photoacoustic tumor imaging

摘要: Abstract Photoacoustic (PA) imaging is a promising modality that provides biomedical information with high sensitivity and resolution. Iron oxide nanoparticles (IONPs) have been regarded as remarkable PA contrast agents because of their low toxicity biodegradable properties. However, IONP delivery restricted by its modest leakage retention in tumors. In this study, we designed IONPs (20nm, 50nm, 100nm) conjugated anti-HER2 moieties [whole IgG, single-chain fragment variable (scFv), peptide] for HER2-targeted tumor imaging. The binding affinity, cellular uptake, vivo biodistribution were examined. We propose 20-nm scFv-conjugated (SNP20) novel agent. SNP20 demonstrated affinity specific to HER2-expressing cells; it selectively visualized HER2-positive tumors studies. These data indicate potential agent From the Clinical Editor be good They may also useful photoacoustic imaging, which can provide image authors here coupled iron antibody showed significant these This combination another option enhanced