The 19 kDa Mycobacterium tuberculosis lipoprotein (LpqH) induces macrophage apoptosis through extrinsic and intrinsic pathways: a role for the mitochondrial apoptosis-inducing factor.
作者： Alejandro Sánchez , Patricia Espinosa , Teresa García , Raúl Mancilla
DOI: 10.1155/2012/950503
关键词: Apoptosis-inducing factor 、 Mitochondrial apoptosis-induced channel 、 Caspase 、 Apoptosis 、 Caspase 3 、 Molecular biology 、 Caspase 8 、 Cell biology 、 Mitochondrion 、 Biology 、 Caspase-9
摘要: We describe the association of caspase-dependent and caspase-independent mechanisms in macrophage apoptosis induced by LpqH, a 19 kDa Mycobacterium tuberculosis lipoprotein. LpqH triggered TLR2 activation, with upregulation death receptors ligands, which was followed receptor signaling cascade activation initiator caspase 8 executioner 3. In this caspase-mediated phase, mitochondrial factors were involved loss transmembrane potential (ΔΨm), release cytochrome c, 9 activation. Interestingly, pathway also identified; immunoblot, inducing factor (AIF) demonstrated nuclei cytosol LpqH-treated macrophages. Confocal microscopy revealed translocation AIF to majority apoptotic cells. These findings emphasize complex redundant nature response mycobacteria.
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