Prospective comparative analysis of the angiogenic capacity of monocytes and CD133+ cells in a murine model of hind limb ischemia.
作者： Natalia López-Holgado , Mercedes Alberca , Fermin Martin Sánchez-Guijo , Eva Maria Villarón , Jose Vicente Rivas
DOI: 10.3109/14653240903191719
关键词: Confocal 、 Laser Doppler velocimetry 、 Pathology 、 Cell 、 Limb perfusion 、 Immunocytochemistry 、 Immunology 、 Blood flow 、 Revascularization 、 Medicine 、 Immunofluorescence
摘要: Background aimsThe aim of this study was to compare prospectively the vasculogenic capacity two cell sources, monocytes and CD133+ cells.MethodsCells were obtained from healthy donors by adherence or magnetic selection. Animals studies performed in a model hind limb ischemia different groups established according type number cells infused. Revascularization measured sequential blood flow analysis using laser Doppler device assessing capillary density ischemic muscles. In order locate infused cells, immunofluorescence immunocytochemistry techniques analyzed light confocal microscopy.ResultsDuring period there significant improvement both perfusion mice treated with either human (P<0.05) compared non-treated mice. No detected as incorporated into vessels when 1 × 105 used but higher doses (1 106) few human...
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