Varicella-Zoster Virus ORF9 Is an Antagonist of the DNA Sensor cGAS
作者： Jonny Hertzog , Rachel E. Rigby , Sonja Roll , Chiara Cursi , Lise Chauveau
DOI: 10.1101/2020.02.11.943415
关键词: Varicella zoster virus 、 Immune system 、 Biology 、 Virology 、 Innate immune system 、 Sting 、 DNA 、 Virus 、 Interferon 、 Transfection
摘要: ABSTRACT Varicella-Zoster virus (VZV) causes chickenpox and shingles. Although infection is associated with severe morbidity in some individuals, the molecular mechanisms that determine innate immune responses remain poorly defined. We found cGAS/STING DNA sensing pathway was critically required for type I interferon (IFN) induction response to VZV infection. Viral gene overexpression screening identified essential tegument protein ORF9 as a novel antagonist of via cGAS. Ectopically well virally expressed bound endogenous Confocal microscopy revealed co-localisation cGAS ORF9, which blocked IFN transfected DNA. also interacted directly cell-free system. Our data further suggest inhibited production cGAMP by Taken together, our work highlights importance recognition an encoded interferes
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