Energy thresholds for ATP synthesis in chloroplasts
作者： Roger P. Hangarter , Norman E. Good
DOI: 10.1016/0005-2728(82)90181-5
关键词: Chloroplast 、 Membrane potential 、 Biophysics 、 Biochemistry 、 ATP synthase 、 Chemistry 、 Chemiosmosis 、 Tricine 、 Pi 、 MOPS 、 HEPES
摘要: Abstract We have investigated the ATP synthesis associated with acid-base transitions in chloroplast lamellae under conditions which allow simultaneous control of thermodynamic variables, ΔpH, membrane potential and ΔGATP. These variables been directly imposed rather than simply inferred. Since initiation labeled Pi incorporation seems to measure accurately net synthesis, following conclusions can be drawn: (1) The proton-motive force is just sufficient for provides almost exactly required energy ΔGATP if efflux three H+ each molecule formed. (2) ΔpH contribute a precisely additive way. Thus, threshold reached or exceeded by absence potential, any combination ΔpH. With large enough occurs even against small inverse In instance combined necessary represent same force.
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