Stochastic simulation of reactive separations in capillary electrophoresis.

摘要: A stochastic (Monte Carlo) simulation is used to investigate thermodynamic and kinetic contributions from the reversible ↔ B reaction in capillary electrophoresis (CE). The effects of equilibrium constant, rate electrophoretic mobility on molecular zone profiles corresponding statistical moments are evaluated. As approaches steady state, velocity governed by constant mobilities reacting molecules. When less than unity, mean more similar that reactant A. Conversely, when greater product B. extent zone-broadening asymmetry at state dependent upon characteristic lifetime, difference between If all other parameters held plate height greatest skew least unity. increases linearly with lifetime difference, whereas independent these parameters. These conclusions have important implications for elucidation information experimental data.