Preparing Protein Solutions for Immunoblotting.

摘要: Most protein solutions can be mixed with sample buffer and analyzed directly by immunoblotting. Any extraction or solvent that is compatible gel electrophoresis used for preparing samples Examples of the suitable immunoblotting include purified proteins, detergent lysates cells, prepared subcellular fractionation, eluates from chromatography columns. The interest must sufficiently abundant in to allow efficient detection, but total amount should not exceed loading capacity gel. For this reason, antigens very low abundance partially mixtures containing high concentrations contaminating proteins. In protocol, are SDS-PAGE boiled prepare electrophoresis.