Insertion and assembly of the precursor of subunit II into the photosystem I complex may precede its processing.

作者: Y. Cohen , J. Steppuhn , R.G. Herrmann , S. Yalovsky , R. Nechushtai

DOI: 10.1002/J.1460-2075.1992.TB05030.X

关键词: BiologyBiophysicsBiogenesisPhotosystem IBiological membranePlastidProtein subunitGene productBiochemistryChloroplastThylakoid

摘要: The biogenesis and assembly of subunit II photosystem I (PSI) (psaD gene product) were studied characterized. precursor the mature form produced in vitro incubated with intact plastids or isolated thylakoids. Following import into plastids, mostly was found However, when processing activity inhibited only present membranes. processed by a stromal peptidase could occur before after insertion thylakoids, both confined to PSI complex. Insertion much less efficient than that precursor. Kinetic studies showed inserted membrane. Only at later stage, began accumulate. These results suggest vivo is embedded as part later, it through action peptidase.

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