Alginate-assisted enrichment and purification of mesenchymal stem cells
作者： Jiao Lin , Xuewen Lu , Puchang Lie , Lingwen Zeng
DOI: 10.1039/C5RA24384J
关键词: Cell biology 、 Cell potency 、 Mesenchymal stem cell 、 Stem cell 、 Biology 、 Progenitor cell 、 Stem cell marker 、 Clinical uses of mesenchymal stem cells 、 Endothelial stem cell 、 Stem cell transplantation for articular cartilage repair
摘要: The umbilical cord contains different populations of stem/progenitor cells such as mesenchymal, hematopoietic, trophoblastic and endothelial cells. However, the isolation methods currently in use are different, resulted liable to cross contamination. Thus it is increasingly difficult compare contrast study outcomes, which hinders progress field stem cell applications. In an attempt address this issue, we have used a magnetism assisted self-assembly method for capturing releasing combination with antibodies mesenchymal antigens, CD105, CD73 CD90, identify localize within placenta cord. This approach capable detecting minimum 10 000 cells, has also shown excellent specificity distinguish other types
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