Evaluation of critical groups required for the binding of heparin to antithrombin

摘要: Abstract We have examined the quantitative importance of various monosaccharide residues an octasaccharide domain heparin that are responsible for binding this oligosaccharide to antithrombin. Different fragments were prepared by enzymatic digestion and avidities these oligosaccharides antithrombin determined equilibrium dialysis. The data show non-reducing-end reducing-end tetrasaccharides contribute equally energy octasaccharide. O6-sulfate group N-acetyl glucosamine moiety within nonreducing-end tetrasaccharide is approximately equal 45% Neither two non-sulfated uronic acid groups flank residue nor N-sulfated on reducing end sequence bears unique O3-sulfate substituent significantly We suggest lack sulfation moieties may be required permit interact with a specific region molecule. However, we cannot exclude possibility plays important role in orienting tetrasaccharide.