Characterization of A Synechocystis Phytochrome

摘要: Phytochromes are photoreceptors common to lower and higher plants. Plant phytochrome apoproteins autocatalytically attach phytochromobilin, a linear tetrapyrrole chromophore [1]. They regulate wide variety of photomorphogenetic responses plants light [2]. The total sequence the Synechocystis sp. PCC 6803 chromosome revealed existence putative gene (phy, ORF slr0473) [3]. phy potentially encodes protein (PhyS) that exhibits homology phytochromes throughout its length, in particular N-terminal region including binding site [4]. Moreover, shows distinct sensory histidine kinases. Sensory kinases so-called response regulators form “two component systems” signal transduction bacteria [5]. Two systems have been discovered more recently also eukaryotes components involved ethylene-induced Arabidopsis [6]. number Several these contain stretches amino acids with limited similarity domain domains ethylene receptor (summarized Table 1). We named “phytochrome like proteins” (abbreviated: plp) [7]. disrupted one plp’s (plpA, encoded by sll1124 [3]) directed insertional mutagenesis. obtained mutant was found be unable grow under blue