High prevalence of Borrelia lusitaniae in Ixodes ricinus ticks in Tunisia.

作者: Hend Younsi , Danièle Postic , Guy Baranton , Ali Bouattour

DOI: 10.1023/A:1010928731281

关键词: IxodidaeNymphAcariBorrelia burgdorferiMicrobiologyBorreliaZoologyIxodesIxodes ricinusBorrelia lusitaniaeMedicine

摘要: To investigate whether ticks of the genus Ixodes are infected by Borrelia burgdorferi complex, 490 unfed ricinus were collected flagging in three different areas Tunisia 1998. DNAs extracted from 81 adults, 60 nymphs and 38 larvae analysed after genic amplification noncoding spacer between two copies rrl–rrf genes B. sl. The prevalence was found to be 34, 33.3 2.6%, respectively. All (n = 61) but one identified as belonging genotypes lusitaniae analysis restriction fragment length polymorphism products. In addition, 290 14 7 used inoculate BSK-H medium isolate spirochetes. Fifteen strains isolated adult humid Tunisia, whereas only obtained larvae. Isolates (15/16) garinii (1/16). These results provide new evidence for existence Lyme borreliosis North Africa.

参考文章(21)
R J Pollack, S R Telford, A Spielman, Standardization of medium for culturing Lyme disease spirochetes. Journal of Clinical Microbiology. ,vol. 31, pp. 1251- 1255 ,(1993) , 10.1128/JCM.31.5.1251-1255.1993
Najeh Lagha, Karim Aoun, Amel Kechrid, Noureddine Bouzouaia, Anissa Zarrouk, La maladie de Lyme en Tunisie, résultats d’une étude clinico-sérologique (1992-1996) Conférence technique de l'Organisation de coopération et de coordination pour la lutte contre les grandes endémies (OCCGE). ,vol. 8, pp. 98- 100 ,(1998)
F Strle, Y Cheng, JA Nelson, MM Picken, JK Bouseman, RN Picken, None, Infection rate of Ixodes ricinus ticks with Borrelia afzelii, Borrelia garinii, and Borrelia burgdorferi sensu stricto in Slovenia. European Journal of Clinical Microbiology & Infectious Diseases. ,vol. 14, pp. 994- 1001 ,(1995) , 10.1007/BF01691382
D. Postic, E. Korenberg, N. Gorelova, Y.V. Kovalevski, E. Bellenger, G. Baranton, Borrelia burgdorferi sensu lato in Russia and neighbouring countries: high incidence of mixed isolates. Research in Microbiology. ,vol. 148, pp. 691- 702 ,(1997) , 10.1016/S0923-2508(99)80068-0
Heike Lottmann, Bettina Wilske, H. Herrmann, Characterization of Borrelia burgdorferi sensu lato strains isolated from Ixodes ricinus in Mecklenburg-Vorpommern, Germany Medical Microbiology and Immunology. ,vol. 184, pp. 181- 184 ,(1996) , 10.1007/PL00008250
M. Cinco, D. Padovan, R. Murgia, L. Frusteri, M. Maroli, I. Pol, N. Verbeek-De Kruif, S. Rijpkema, F. Taggi, Prevalence of Borrelia burgdorferi infection in Ixodes ricinus in central Italy. European Journal of Clinical Microbiology & Infectious Diseases. ,vol. 17, pp. 134- 135 ,(1998) , 10.1007/BF01682174
Alan G. Barbour, Willy Burgdorfer, Stanley F. Hayes, Olivier Péter, André Aeschlimann, Isolation of a cultivable spirochete from Ixodes ricinus ticks of Switzerland Current Microbiology. ,vol. 8, pp. 123- 126 ,(1983) , 10.1007/BF01566969
Leonas Motiejunas, Jonas Bunikis, Alan G. Barbour, Ariadna Sadziene, Lyme Borreliosis in Lithuania Scandinavian Journal of Infectious Diseases. ,vol. 26, pp. 149- 155 ,(1994) , 10.3109/00365549409011778