Determination of fungal activity in modified wood by means of micro-calorimetry and determination of total esterase activity

作者: Pradeep Verma , Jens Dyckmans , Holger Militz , Carsten Mai

DOI: 10.1007/S00253-008-1525-Z

关键词: EsteraseCalorimetryEnzyme assayFood scienceFungal proteinHydrolysisIsothermal microcalorimetryBiochemistryTrametes versicolorUreaChemistry

摘要: Beech and pine wood blocks were treated with 1,3-dimethylol-4,5-dihydroxyethylen urea (DMDHEU) to increasing weight percent gains (WPG). The resistance of the specimens against Trametes versicolor Coniophora puteana, determined as mass loss, increased WPG DMDHEU. Metabolic activity fungi in was assessed total esterase (TEA) based on hydrolysis fluorescein diacetate heat or energy production by isothermal micro-calorimetry. Both methods revealed that fungal related loss caused fungi. Still, detected even highest showed treatment not toxic Energy a higher consistency after decay than TEA; more stringently reflected rate. Heat did proceed linearly, possibly due inhibition an excess carbon dioxide.

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