Structural Characterization of a Novel Polysaccharide from Lepidium meyenii (Maca) and Analysis of Its Regulatory Function in Macrophage Polarization in Vitro
作者： Mengmeng Zhang , Wenjia Wu , Yao Ren , Xiaofeng Li , Yuqian Tang
关键词: Congo red 、 Chemistry 、 Arabinose 、 Polysaccharide 、 Macrophage polarization 、 Mannose 、 Lepidium meyenii 、 Galactose 、 In vitro 、 Biochemistry
摘要: In our previous study, three novel polysaccharides, named MC-1, MC-2, and MC-3, were separated from the roots of maca (Lepidium meyenii), which is a food source Andes region. The structural information immunomodulatory activity MC-1 then investigated. structure MC-2 are still unknown. this characterization revealed that has an average molecular weight 9.83 kDa composed arabinose (20.9%), mannose (4.5%), glucose (71.9%), galactose (2.7%). main linkage types proven to be (1→5)-α-l-Ara, (1→3)-α-l-Man, (1→)-α-d-Glc, (1→4)-α-d-Glc, (1→6)-α-d-Glc, (1→6)-β-d-Gal by methylation NMR analyses. Congo red assay showed possesses triple-helix conformation. Immunostimulating assays indicated could induce M1 polarization original macrophages convert M2 into phenotype. Although not shift M2, it inhibit inflammatory reactions indu...
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