Radiotherapy Combined with Gemcitabine and Oxaliplatin in Pancreatic Cancer Cells

摘要: Clinical evidence suggests that gemcitabine (Gem) plus oxaliplatin (Ox) is superior to alone in advanced pancreatic carcinoma. The addition of radiation improves response and a standard treatment for locally disease. We investigated the effect on gemcitabine-based chemoradiation by determining whether produced synergistic cytotoxicity using median analysis radiosensitization clonogenic survival assays. analyzed effects cell cycle distribution DNA content radiation-induced damage repair phosphorylated H2AX (γ-H2AX). Gemcitabine schedule-dependent BxPC-3 Panc-1 cells (combination indices: 0.76 ± 0.05, 0.61 0.11). In cells, did not affect gemcitabine-mediated (Gem 1.99 0.27; Gem + Ox 2.38 0.30). significantly enhanced 1.31 0.05; 2.90 0.31). Radiosensitization was accompanied early S-phase arrest induction/persistence γ-H2AX protein, which were unaltered oxaliplatin. Addition produces equal or greater than alone, supporting our clinical investigation with gemcitabine-radiation cancer aimed at improving systemic disease control while maintaining local tumor radiosensitization.