Double antibody for enhanced sensitivity in immunoassay

摘要: Method and compositions are provided for performing homogeneous immunoassays. The method involves having a signal producing system, which provides detectable signal, system includes macromolecular member. determination of the analyte, is member specific binding pair consisting ligand its homologous receptor, performed by creating an extensive matrix in assay medium addition to labeled with one members antiligand either present as analyte or added, polyvalent receptor antiligand, any additional system. ligand, create modulates, e.g. inhibits, approach ligand. extent degree formation dependent upon concentration medium. By comparing from unknown amount obtained known sample may be determined qualitatively quantitatively. Kits predetermined amounts various reagents allow enhanced sensitivity method.