The SAGA complex in the rice pathogen Fusarium fujikuroi: structure and functional characterization

摘要: Post-translational modification of histones is a crucial mode transcriptional regulation in eukaryotes. A well-described acetylation modifier certain lysine residues the Spt-Ada-Gcn5 acetyltransferase (SAGA) complex assembled around histone Gcn5 Saccharomyces cerevisiae. We identified and characterized SAGA rice pathogen Fusarium fujikuroi, well-known for producing large variety secondary metabolites (SMs). By using co-immunoprecipitation approach, almost all S. cerevisiae components have been identified, except ubiquitinating DUBm module chromodomain containing Chd1. Deletion GCN5 led to impaired growth, loss conidiation alteration SM biosynthesis. Furthermore, we show that essential several 3 lysines F. i.e. H3K4, H3K9, H3K18 H3K27. genome-wide microarray analysis revealed differential expression about 30% genome with an enrichment genes involved primary metabolism, transport modification. HPLC-based known SMs significant alterations Δgcn5 mutant. While most were activated by activity, biosynthesis pigment bikaverin was strongly increased upon deletion underlining diverse roles fujikuroi. This article protected copyright. All rights reserved.