Gene amplification at a locus encoding a putative Na+/H+ antiporter confers sodium and lithium tolerance in fission yeast.

摘要: Abstract We have identified a new locus, sodium 2 (sod2) based on selection for increased LiCl tolerance in fission yeast, Schizosaccharomyces pombe. Tolerant strains enhanced pH-dependent Na+ export capacity and transport experiments suggest that the gene encodes an Na+/H+ antiport. The predicted sod2 product can be placed broad class of transporters which possess 12 hydrophobic transmembrane domains. protein shows some sequence similarity to human bacterial antiporters. Overexpression conferred tolerance. Osmotolerance was not affected cells were unaffected growth K+. In disruption strain incapable exporting sodium. They hypersensitive or Li+ could grow under conditions approximate pH7. amplification selected stepwise degree such correlated with level