Calculation of resonance energy transfer in crowded biological membranes

摘要: Analytical and numerical models were developed to describe fluorescence resonance energy transfer (RET) in crowded biological membranes. It was assumed that fluorescent donors linked membrane proteins acceptors lipids. No restrictions placed on the location of donor within protein or partitioning between two leaflets bilayer; however, excluded from area occupied by proteins. equations derived give average quantum yield a at low concentrations. Monte Carlo simulations used generate lipid distributions numerically with RET determine distribution lifetimes high concentrations, up 50% fraction. The results show such crowding always reduces yield, probably because increases acceptor concentrations near donor-bearing proteins; magnitude reduction monotonically concentration. also can differ markedly, even for systems possessing same lifetime. dependence concentration, radius, position protein, fraction each leaflet examined. model are directly applicable analysis data obtained membranes; their application should result more complete accurate determination structures components.