Method of selection, by two-dimensional separation, of nucleic acids that bind to a target with high affinity

摘要: The invention relates to a method of selection, by two-dimensional separation, nucleic acids that bind target molecule with high affinity from mixture acids. inventive comprises the following steps: a) subjecting physico-chemical separation step, thereby obtaining set mixed fractions containing acids, run parameter window being associated every fraction b) contacting molecule, binding acid/target complexes, and c) step same as in a), selecting complexes whose parameters are outside window.