Method for the production of baccatin-III by enzymatic synthesis

摘要: A method for the continuous synthesis of baccatin-III is performed by means enzymatic acetylations 10-deacetyl (10-DAB) in an enzyme reactor. This objective accomplished a method, which aqueous reaction batch used, contains partially purified or very pure acetyl transferase from Taxus species and components, especially 10-DAB, compounds optionally coenzyme A, can be regenerated, protective colloid buffer substance solution. The solution separated semipermeable membrane organic solvent, serves as extraction agent baccatin-III, being regenerated at regular intervals system solvent continuously exchanged. By conducting reaction, product removed partial taxol taxoter. above achievable yields are increased significantly.