Regeneration of Dopaminergic Neurons in Adult Zebrafish Depends on Immune System Activation and Differs for Distinct Populations

摘要: Adult zebrafish, in contrast to mammals, regenerate neurons their brain, but the extent and variability of this capacity is unclear. Here we ask whether loss various dopaminergic neuron populations sufficient trigger functional regeneration. Both sexes zebrafish were analyzed. Genetic lineage tracing shows that specific diencephalic ependymo-radial glial (ERG) progenitor cells give rise new [tyrosine hydroxylase-positive (TH+)] neurons. Ablation elicits an immune response, increased proliferation ERG cells, addition TH+ constitutively add (e.g., population 5/6). Inhibiting response attenuates neurogenesis control levels. Boosting enhances proliferation, not In contrast, which constitutive undetectable posterior tuberculum locus ceruleus), cell replacement tissue integration are incomplete transient. This associated with a spinal axons, as well permanent deficits shoaling reproductive behavior. Hence, adult brain show vast differences regenerative correlate depend on system activation. SIGNIFICANCE STATEMENT Despite fact high propensity study reveals all types functionally regenerated after ablation. same vertebrate mechanisms successful regeneration can be studied. We identify for activating promotes these cells. However, some areas only leads insufficient important later disappear. Understanding may inform interventions targeting neurons, such from endogenous nonregenerating mammals.