Salt-Gradient Approach for Regulating Capture-to-Translocation Dynamics of DNA with Nanochannel Sensors

摘要: Understanding the physical mechanisms that govern ion and fluidic transport in salt-concentration-based nanochannel/nanopore systems is essential for potential applications bioanalysis. One central challenge to interpret observed four-stage change from osmosis reverse one with increasing salt gradient. Here we provide a unified model outlines intriguing role of two competing factors, exclusion- diffusion-induced electrical potentials. We demonstrate theoretically direction control hydrodynamic flow via Based on this, also propose salt-gradient approach regulating DNA motion nanochannels enables voltage-free single-molecule capture significantly low translocation speed. The present method would be used as useful protocol overcome key hurdle tailoring capture-to-translocation dynamics polynucleotides nanopore sequencing.