Some properties of the polygalacturonase from four Zimbabwean wild fruits (Uapaca kirkiana, Zizphus mauritiana, Tamarindus indica and Berchemia discolor fruits)

摘要: Abstract Polygalacturonase was extracted from ripe Uapaca kirkiana , Zizphus mauritiana Tamarindus indica and Berchemia discolor fruits of Zimbabwe. Protein concentrations activities the enzymes in extracts were determined four fruit extracts. The protein enzyme ranged 0.82 ± 0.17 to 1.97 ± 0.13 mg/ml 1.99 ± 0.13 6.64 ± 0.38 mmol min −1  mg Optimum pH 4.5 5 optimum temperature 25 37 °C. reduced viscosity 1% pectin solutions an experiment which done together with assay for reducing sugars prove wild fruits. K m V max 0.115 0.252 0.0057 0.0119 mmol groups/min/mg protein, respectively, polygalacturonase Calcium chloride sodium activated PG all sources a greater extend than magnesium barium chloride. other three had very little effect on polysaccharide U. .