MiR-429 suppresses proliferation and invasion of breast cancer via inhibiting the Wnt/β-catenin signaling pathway.

摘要: BACKGROUND microRNAs (miRNAs) have been verified as molecular targets for regulating tumor proliferation, invasion, and metastasis in progression. However, the relationship between miRNAs cellular energy metabolism breast cancer still needs to be clarified. This study aimed investigate role of miR-429 METHODS Bioinformatic analyses were employed detect cancer-related signaling pathways. We used a Kaplan-Meier curve analyze survival rate patients with high or low expression miR-429. real-time quantitative PCR (RT-qPCR) different cell lines. Sh-con, over-miR-429, inhibitor, sh-inhibitor control transfected. Colony formation EDU assay proliferation transfected cells. Wound healing transwell assays performed mobility invasion ability Western blot was relative protein cells tissues. conducted target proteins databases. Dual luciferase reporter confirm binding site fibronectin 1 (FN1). RESULTS The results our indicate that MiR-429 its genes are associated pathways higher corresponds better prognosis. When overexpressed, MDA-MB-231 inhibited. able suppress Wnt/β-catenin pathway, FN1 overexpression could rescue influence over-miR-429. CONCLUSIONS suggest suppresses via inhibiting pathway.