Differential binding of mutant glucocorticoid receptors to the glucocorticoid response element of the tyrosine aminotransferase gene.

摘要: Glucocorticoid receptors (GCRs) in sublines of the mouse P1798 lymphosarcoma that are sensitive (S) or resistant (R) to glucocorticoid-induced cell lysis were examined for their ability bind a single glucocorticoid responsive element (GRE). Mobility shift assays detected two specific complexes identical both S and R cellular extracts. Antibodies against GCR N-terminus supershifted complexes, suggesting 97 kDa wild-type (WT-GCR) cells, variant, non-steroid-binding (NSB-GCR) cells components complexes. Sephacryl S300 gel filtration column fractions containing WT-GCR NSB-GCR formed with GRE, while second variant 45 steroid-binding truncated (TR-GCR), did not. Southwestern blotting GRE-binding, protein band A was not detected. UV crosslinking DNA revealed range 92-120 crosslinked GRE No at kDa. Strong interaction GREs lack binding TR-GCR illustrate complex receptor system lymphosarcoma.